Insertional specificity of transposon Tn5 in Acinetobacter sp
نویسندگان
چکیده
منابع مشابه
Specificity of transposon Tn5 insertion.
Genetic mapping studies had shown that the bacterial transposon Tn5 can insert into many sites in a gene, but that some sites are preferred. To begin understanding Tn5's insertion specificity at the molecular level, we selected transpositions of Tn5 from the Escherichia coli chromosome to the plasmid pBR322 and analyzed the resultant pBR322::Tn5 plasmids by restriction endonuclease digestion an...
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Tn5 is a composite bacterial transposon that encodes a protein, transposase (Tnp), required for movement of the transposon. The initial step in the transposition pathway involves specific binding of Tnp to 19-bp end recognition sequences. Tn5 contains two different specific end sequences, termed outside end (OE) and inside end (IE). In Escherichia coli, IE is methylated by Dam methylase (IE(ME)...
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The bacterial transposon Tn5 encodes two proteins, the transposase and a related protein, the transposition inhibitor, whose relative abundance determines, in part, the frequency of Tn5 transposition. The synthesis of these proteins is programmed by a complex set of genetic regulatory elements. The host DNA methylation function, dam, inhibits transposase promoter recognition and indirectly enha...
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Transposable elements induce spontaneous mutations, promote genome rearrangements, regulate gene expression, and participate in the horizontal spread of genes encoding traits such as antibiotic resistance among bacterial genera too distantly related to undergo homologous recombination. Here we review the bacterial transposon Tn5 and focus on those aspects of its functional organization and tran...
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Plant Research International, P.O. Box 16, 6700 AA Wageningen, The Netherlands (R.G., A.P); Institute of Molecular Plant Sciences Leiden University, The Netherlands (P.B.F.O., J.H.C.H); Centre de Coopération Internationale en Recherche Agronomique pour le Développement, Montpellier, France (C.S., E.G.); John Innes Centre, Norwich, United Kingdom (A.K., P.C.); University of Milan, Italy (L.C.); ...
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ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 1984
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.157.2.607-611.1984